KiPS c1

General#

Cell Line

hPSCreg Name CAMi001-A
Alternative name(s)
KiPS c1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator University of Cambridge (CAM)

External Databases

Cellosaurus CVCL_2Z89

General Information

Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Contact information / weblink
Alternatives to consent
Alternative consent approval number
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Details on restriction to research project
Policy for access to genetic information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Name of accrediting authority involved?
Approval number
Name of accrediting authority involved?
Approval number
Please describe:
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation#

General

Source cell type
keratinocyte
An epidermal cell which synthesizes keratin and undergoes a characteristic change as it moves upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
Synonyms
  • malpighian cell
  • keratinized cell of epidermis
Age of donor (at collection) 35-39

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
200 mM L - Gulutamine (x100) 1 mM
Non-essential amino acid x100
2Mercaptoethanol 0.1 mM
bFGF 10 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Unkown
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
ZFP42 (REX-1)
Unkown
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)