Cell Line

hPSCreg Name CAMi005-B
Alternative name(s)
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th August 2019
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Generator University of Cambridge (CAM)

External Databases

Cellosaurus CVCL_9S02

General Information

* Is the cell line readily obtainable for third parties?

Donor Information#

General Donor Information

Sex unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor


Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink
Alternatives to consent
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Name of accrediting authority involved? Lothian Healthcare Trust
Approval number
Name of accrediting authority involved? Lothian Healthcare Trust
Approval number
Please describe:
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation#


Source cell type (free text) Foetal neural stem cells

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
Absence of reprogramming vector(s)?
Reprogramming vectors silenced?

Vector free reprogramming


Derived under xeno-free conditions
Derived under GMP?
Available as clinical grade?

Culture Conditions#

Medium mTeSR™ 1
DMEM-F12(invitrogen 21331-020) 500 ml
NON-ESSENTIAL AMINO ACID (x100) (invitrogen 11140) 6.3 ml
200 mM L-GULUTAMINE(x100) (invitrogen 25030) 6.3 ml
KSR(final 20%)(invitrogen 10828) 125 ml
2-MERCAPTOETHANOL(x1000stock)(final 0.1 mM) 630 µl
bFGF (Peprotech 315-09final 10ng/ml)


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
TRA 1-60
TRA 1-81
Differentiation Potency
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Karyotyping method: G-Banding

Other Genotyping (Cell Line)