2F8

General#

Cell Line

hPSCreg Name CAMi005-B
Alternative name(s)
2F8
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator University of Cambridge (CAM)

External Databases

Cellosaurus CVCL_9S02

General Information

Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type (free text) Foetal neural stem cells

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium mTeSR™ 1
Supplements
DMEM-F12(invitrogen 21331-020) 500 ml
NON-ESSENTIAL AMINO ACID (x100) (invitrogen 11140) 6.3 ml
200 mM L-GULUTAMINE(x100) (invitrogen 25030) 6.3 ml
KSR(final 20%)(invitrogen 10828) 125 ml
2-MERCAPTOETHANOL(x1000stock)(final 0.1 mM) 630 µl
bFGF (Peprotech 315-09final 10ng/ml)
penicillin/streptomysin

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Unkown
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)