General

Cell Line
hPSCreg name	CHOPi014-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	CHOPi014-A
Alternative name(s)	CHOPWT15
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	5th August 2025
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Provider
Generator	The Children's Hospital of Philadelphia (CHOP)
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed

Donor Information

General Donor Information
Sex	male
Ethnicity	Black
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Is the medical history available upon request?	NoNo

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	No
Do you (Depositor/Provider) hold the original Donor Consent Form?	No
If you do not hold the Donor Consent Form, do you know who does?	Yes
Confirm that consent was obtained by a qualified professional	Yes
Has the donor agreed to be re-contacted?	No
Has the donor been informed about how her/his data will be protected?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
* Does consent expressly prevent the derivation of pluripotent stem cells?	No
* Does consent pertain to a specific research project?	No
Does consent permit unforeseen future research, without further consent?	Yes
Does consent expressly prevent development of commercial products?	No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
Does consent permit research by
an academic institution?	Yes
a non-profit company?	Yes
How may genetic information associated with the cell line be accessed?	No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	Yes
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	SeraTrials, LLC, A BioreclamationIVT Company
Approval number	WIRB® Protocol #20161665
Do you have obligations to third parties in regard to the use of the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Peripheral Blood Mononuclear Cell A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes. Synonyms PERIPHERAL BLOOD MONONUCLEAR CELL Peripheral Blood Mononuclear Cell PBMC
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Is reprogramming vector detectable?	No
Methods used	RT-PCR
Vector free reprogramming
Other
Selection criteria for clones	Morphology and sendai Clearance
Derived under xeno-free conditions	Yes
Derived under GMP?	No
Available as clinical grade?	No

Culture Conditions

Surface coating	Gelatin
Feeder cells	MEF
Passage method	Enzymatically TrypLE
O2 Concentration	5 %
CO2 Concentration	5 %
Medium	Other medium: Base medium: HES10 Main protein source: Knock-out serum replacement Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
SSEA-3	Yes
SSEA-4	Yes
SSEA-1	No
TRA 1-60	Yes
TRA 1-81	Yes

Differentiation Potency

Endoderm

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes Normal Karyotyping method: G-Banding
Other Genotyping (Cell Line)