ARSM44081223
CHUQi002-A
General
Cell Line |
|
| hPSCreg name | CHUQi002-A |
| Cite as: | CHUQi002-A |
| Alternative name(s) |
ARSM44081223
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 18th November 2024 |
| User feedback | |
Provider |
|
| Generator | CHU de Québec-Université Laval Research Center (CHUQ) |
| Derivation country | Canada |
External Databases |
|
| BioSamples | SAMEA115975574 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA115975575 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | Nicolas.dupre@fmed.ulval.ca |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Alternatives to consent are available? | No |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | Yes |
| Has the donor been informed about how her/his data will be protected? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Comité d’éthique de la recherche de l’Hôpital de l’Enfant-Jésus du CHU de Québec |
| Approval number | 2012-1286 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell line name | ARSM44081223 |
| Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
|
| Source cell origin |
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
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Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Notes on reprogramming vector detection | Real-time PCR detection with pre-design TaqMan assays from ThermoFIsher |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
Small molecules
|
| Small molecules |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
|||||
| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
|||||
| SSEA-4 |
Yes |
|||||
| TRA 1-60 |
Yes |
|||||
| TRA 1-81 |
Yes |
Morphology pictures
ARSM44081223 clone3 P5 85% J3.tif
Morphology at P5, 3 days after thawing
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
In vitro spontaneous differentiation
Scorecard
| Marker | Expressed |
| Somatostatin |
Yes |
| claudin-1 |
Yes |
Protocol or reference
MAN0008384_TaqMan_hPSC_Scorecard_Panel_UG.pdf
Original Scorecard protocol
In vitro spontaneous differentiation
Scorecard
| Marker | Expressed |
| hes related family bHLH transcription factor with YRPW motif 1 |
Yes |
| T-box transcription factor 3 |
Yes |
Protocol or reference
MAN0008384_TaqMan_hPSC_Scorecard_Panel_UG.pdf
Original Scorecard protocol
In vitro spontaneous differentiation
Scorecard
| Marker | Expressed |
| paired box 6 |
Yes |
| nuclear receptor subfamily 2 group F member 2 |
Yes |
Protocol or reference
MAN0008384_TaqMan_hPSC_Scorecard_Panel_UG.pdf
Original Scorecard protocol
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
5-7
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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