ARCAM?
The cell line is not validated yet.
CHUQi013-A
General
Cell Line |
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| hPSCreg name | CHUQi013-A |
| Cite as: | CHUQi013-A |
| Alternative name(s) |
ARCAM?
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 14th May 2026 |
| User feedback | |
Provider |
|
| Generator | CHU de Québec-Université Laval Research Center (CHUQ) |
| Derivation country | Canada |
External Databases |
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| BioSamples | SAMEA122587875 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
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| BioSamples | SAMEA122587876 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| * Does consent pertain to a specific research project? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | CER du CHU de Québec-Université Laval |
| Approval number | 2012-1286 (PEJ-235) |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell line name | ARCAM?241022 |
| Source cell type |
A mixture of monocytes and lymphocytes; blood leucocytes from which granulocytes have been separated and removed.
Synonyms
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| Source cell origin |
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
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| Collected in | 2022 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Notes on reprogramming vector detection | qRT-PCR |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
Small molecules
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| Small molecules |
|
Other |
|
| Selection criteria for clones | by morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-60 |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
Morphology pictures
Morphology after 4 days in culture
qRT-PCR on more markers of undifferentiated stem cell
Method documentation
Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
Protocol or reference
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
| Marker | Expressed |
| CD140b |
Yes |
| CD144 |
Yes |
| hes related family bHLH transcription factor with YRPW motif 1 |
Yes |
| T-box transcription factor 3 |
Yes |
Protocol or reference
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
| Marker | Expressed |
| SOX2 |
Yes |
| paired box 6 |
Yes |
| nuclear receptor subfamily 2 group F member 2 |
Yes |
| paired box 3 |
Yes |
Protocol or reference
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
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