CW70037

General

Cell Line

hPSCreg name CIRMi294-A
Cite as:
CIRMi294-A (RRID:CVCL_4S73)
Alternative name(s)
CW70037
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
CIRMi499-A
(CW70314)
Donor diseases:
type 1 diabetes mellitus
CIRMi505-A
(CW70320)
Donor diseases:
type 1 diabetes mellitus
CIRMi528-A
(CW70353)
Donor diseases:
type 1 diabetes mellitus
CIRMi538-A
(CW70369)
Donor diseases:
type 1 diabetes mellitus
CIRMi286-A
(CW70028)
Donor diseases:
type 1 diabetes mellitus
CIRMi287-A
(CW70029)
Donor diseases:
type 1 diabetes mellitus
CIRMi373-A
(CW70143)
Donor diseases:
type 1 diabetes mellitus
CIRMi526-A
(CW70350)
Donor diseases:
type 1 diabetes mellitus
CIRMi419-A
(CW70208)
Donor diseases:
type 1 diabetes mellitus
CIRMi307-A
(CW70052)
Donor diseases:
type 1 diabetes mellitus
CIRMi451-A
(CW70254)
Donor diseases:
type 1 diabetes mellitus
ASGRCi002-A
(F3-3#1 CHR)
Donor diseases:
Type 1 Diabetes Mellitus
ASGRCi003-A
(F3-4#5 CHR)
Donor diseases:
Type 1 Diabetes Mellitus
ESi065-A
([IDDM1] FiPS 1.13-Ep6F-9)
Donor diseases:
obsolete_type I diabetes mellitus
CIRMi466-A
(CW70270)
Donor diseases:
type 2 diabetes mellitus
CIRMi467-A
(CW70272)
Donor diseases:
type 2 diabetes mellitus
CIRMi470-A
(CW70275)
Donor diseases:
type 2 diabetes mellitus
CIRMi475-A
(CW70281)
Donor diseases:
type 2 diabetes mellitus
CIRMi136-A
(CW50050)
Donor diseases:
type 2 diabetes mellitus
CIRMi477-A
(CW70283)
Donor diseases:
type 2 diabetes mellitus
CIRMi478-A
(CW70284)
Donor diseases:
type 2 diabetes mellitus
CIRMi486-A
(CW70298)
Donor diseases:
type 2 diabetes mellitus
CIRMi490-A
(CW70303)
Donor diseases:
type 2 diabetes mellitus
CIRMi491-A
(CW70304)
Donor diseases:
type 2 diabetes mellitus
CIRMi495-A
(CW70308)
Donor diseases:
type 2 diabetes mellitus
CIRMi500-A
(CW70315)
Donor diseases:
type 2 diabetes mellitus
CIRMi502-A
(CW70317)
Donor diseases:
type 2 diabetes mellitus
CIRMi503-A
(CW70318)
Donor diseases:
type 2 diabetes mellitus
CIRMi165-A
(CW50093)
Donor diseases:
type 2 diabetes mellitus
CIRMi510-A
(CW70325)
Donor diseases:
type 2 diabetes mellitus
CIRMi516-A
(CW70338)
Donor diseases:
type 2 diabetes mellitus
CIRMi390-A
(CW70174)
Donor diseases:
type 2 diabetes mellitus
CIRMi391-A
(CW70175)
Donor diseases:
type 2 diabetes mellitus
CIRMi520-A
(CW70343)
Donor diseases:
type 2 diabetes mellitus
CIRMi523-A
(CW70346)
Donor diseases:
type 2 diabetes mellitus
CIRMi400-A
(CW70080)
Donor diseases:
type 2 diabetes mellitus
CIRMi532-A
(CW70358)
Donor diseases:
type 2 diabetes mellitus
CIRMi281-A
(CW70017)
Donor diseases:
type 2 diabetes mellitus
CIRMi536-A
(CW70366)
Donor diseases:
type 2 diabetes mellitus
CIRMi282-A
(CW70018)
Donor diseases:
type 2 diabetes mellitus
CIRMi537-A
(CW70368)
Donor diseases:
type 2 diabetes mellitus
CIRMi539-A
(CW70370)
Donor diseases:
type 2 diabetes mellitus
CIRMi540-A
(CW70371)
Donor diseases:
type 2 diabetes mellitus
CIRMi543-A
(CW70384)
Donor diseases:
type 2 diabetes mellitus
CIRMi291-A
(CW70034)
Donor diseases:
type 2 diabetes mellitus
CIRMi297-A
(CW70040)
Donor diseases:
type 2 diabetes mellitus
CIRMi299-A
(CW70042)
Donor diseases:
type 2 diabetes mellitus
CIRMi434-A
(CW70230)
Donor diseases:
type 2 diabetes mellitus
CIRMi452-A
(CW70255)
Donor diseases:
type 2 diabetes mellitus
CIRMi454-A
(CW70257)
Donor diseases:
type 2 diabetes mellitus
Last update 12th June 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator California Institute for Regenerative Medicine (CIRM)
Owner California Institute for Regenerative Medicine (CIRM)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA113585374
Cellosaurus CVCL_4S73
Wikidata Q54816742

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 25-29

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
DIABETES TYPE I
Synonyms
  • IDDM
  • diabetes mellitis type 1
  • diabetes mellitis type I
  • immune mediated diabetes
  • insulin dependent diabetes
  • insulin-dependent diabetes mellitus
  • juvenile diabetes
  • type 1 diabetes
  • type I diabetes
  • type I diabetes mellitus
  • T1D
  • T1DM
show more synonyms

External Databases (Donor)

BioSamples SAMEA113585375

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. info@cirm.ca.gov
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information info@cirm.ca.gov
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? UC San Diego
Approval number #140090
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? UC San Diego
Approval number #140090
Is there an MTA available for the cell line? Yes

hIPSC Derivation

General

Source cell type
A cell found predominately in the blood.
Age of donor (at collection) 25-29

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
Lin28
Yes
Undifferentiated cells were analyzed by proprietary assays approved by California Institute of Regenerative Medicine (CIRM) as described in the attached publication. Pluripotency of established iPSC cultures were tested by qPCR on bulk samples using 48 genes including standard pluripotency genes (listed above). Samples were assigned a passing score after analysis with a non-supervised classifier based on an appropriate sample set. (Novak, T. 2015. Drug Discovery World pp. 47-53.)
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Mesoderm
Ont Id: UBERON_0000926
Ectoderm
Ont Id: UBERON_0000924

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal

Other Genotyping (Cell Line)