iFCI027
CRICKi002-A
General
Cell Line |
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| hPSCreg name | CRICKi002-A |
| Cite as: | CRICKi002-A (RRID:CVCL_A5EV) |
| Alternative name(s) |
iFCI027
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 2nd November 2024 |
| User feedback | |
Provider |
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| Generator | The Francis Crick Institute Limited (CRICK) |
| Owner | The Francis Crick Institute Limited (CRICK) |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
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| Cellosaurus | CVCL_A5EV |
| Wikidata | Q107114722 |
| BioSamples | SAMEA13315508 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
the cell line can be obtained by third parties using appropriate MTA |
Donor Information
General Donor Information |
|
| Sex | unknown |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA13315606 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | Cristina.dias@kcl.ac.uk |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | LONDON-CAMDEN AND KINGS CROSS RESEARCH ETHICS COMMITTEE |
| Approval number | 17/LO/0981 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
hIPSC Derivation
General |
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| Source cell type |
An immature, nucleated erythrocyte occupying the stage of erythropoiesis that follows formation of erythroid progenitor cells and precedes formation of reticulocytes.
Synonyms
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| Source cell origin | |
| Passage number reprogrammed | 1 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Laminin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
Differentiation Potency
In vitro spontaneous differentiation
Scorecard
| Marker | Expressed |
| PAX6 |
Yes |
| BETA III TUBULIN |
Yes |
Morphology
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
NO CHROMOSSOMAL ABERRATIONS
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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