General

iPSC Line
hPSCreg name	CVTTHi001-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	CVTTHi001-A-1
iPSC line type	Human induced pluripotent stem cell (hiPSC)
Similar iPSC lines	CVTTHi001-A Donor diseases: Activated PI3K-delta syndrome CHCMUi001-A Donor diseases: Activated PI3K-delta syndrome
Last update	24th September 2024
User feedback show/hide	Written by on Delete No feedback available yet. Login to share your feedback, experiences or results with the research community.
Provider
Generator	Centro Vasco de Transfusión y Tejidos Humanos (CVTTH)
External iPSC Databases
BioSamples	SAMEA115903643
General iPSC Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	CVTTHi001-A

iPSC Genetic Modification

Disease/phenotype related modifications	Genetic modifications PIK3R1 (target)
Genetic modifications not related to a disease	Type of iPSC Modification Gene knock-in Chromosome location chr 5q13.1 Delivery method CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Activated PI3K-delta syndrome APD syndrome type 2 (mutations in the PIK3R1 gene) Synonyms APDS Senescent T-cells-lymphadenopathy-immunodeficiency syndrome due to p110delta-activating mutation Activated p110-delta syndrome PASLI PI3K delta activating mutations causing senescent T cells, lymphadenopathy and immunodeficiency show more synonyms show less synonyms
External Databases (Donor)
BioSamples	SAMEA7274737

Ethics

Also have a look at the ethics information for the parental line CVTTHi001-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

iPSC Derivation

General
The source cell information can be found in the parental cell line CVTTHi001-A.
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

iPSC Culture Conditions

Medium

Essential 8™

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
POU5F1 (OCT-4)	Yes	–
SSEA-4	Yes	–

Morphology

Morphology pictures

Morphology.tif

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro directed differentiation

Morphology

Trilineage differentiation.tif

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro directed differentiation

Morphology

Trilineage differentiation.tif

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro directed differentiation

Morphology

Trilineage differentiation.tif

Genotyping

Karyotyping (iPSC Line)

Other Genotyping (iPSC Line)