DRICUi009-A

ADLON44426UC

General

Cell Line

hPSCreg Name
DRICUi009-A
Alternative name(s)
ADLON44426UC
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th January 2023
Notes Further anonymized information about the donor including demographic and clinical data will be made available on the Dementia Platforms UK data portal: https://portal.dementiasplatform.uk/
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator UK Dementia Research Institute, Cardiff University (DRICU)
Owner UK Dementia Research Institute, Cardiff University (DRICU)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA10291416
Cellosaurus CVCL_B3P7
Wikidata Q110432788

General Information

* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Disease research
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA10563839

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? Yes
Details on restriction to research project Late Onset Alzheimer's Disease Genetic Resource, Kings College London
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: Relevant clinical information will be made available via a website hosted by Dementias Platform UK
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? MREC for Wales
Approval number REC ID 00/09/42
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved?
Approval number
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? Yes
Further constraints on use Use is restricted to disease research
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Thermo Fisher is the supplier of CytoTune™-iPS 2.0 Sendai Reprogramming Kit, used for iPSC reprogramming
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution No commercial use, as described in the Limited use label License No: 518 CytoTune™ Technology for Products, owned by DNAVEC Corporation

hIPSC Derivation

General

Source cell type
T-Lymphocyte
A thymocyte-derived lymphocyte of immunological importance that is long-lived (months to years) and is responsible for cell-mediated immunity. T lymphocyte cells form rosettes with sheep erythrocytes and, in the presence of transforming agents (mitogens), differentiate and divide. These cells have the characteristic T3 surface marker and may be further divided into subsets according to function, such as helper, cytotoxic, etc.
Synonyms
  • T Cells
  • T Cell
  • T-Lymphocytes
  • Thymus-Dependent Lymphocytes
  • T cell
  • T-Lymphocyte
  • T-Cell
  • T lymphocyte
  • Thymus Derived Lymphocyte
  • T-Cells
show more synonyms
Source cell origin
Blood
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection Virus undetectable at passage 8
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Advanced DMEM/F12 (Gibco Thermo Fisher 12634010)
Main protein source: Albumine
Serum concentration: 0 %
Supplements
GlutaMAX (Gibco Thermo Fisher 35050061) 1 %
Heparin (Stem Cell Technologies 07980) 0.1 µg/ml
Ascorbic Acid-2-phosphate magnesium salt (Sigma A8960) 0.22 mM
HEPES 1M pH 7.4 (Gibco Thermo Fisher 15630080) 15 mM
FGF-2 basic 145aa (Miltenyi Biotech 130-093-842) 100 ng/ml
TGF-beta (Biolegend 781804) 2 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
Morphology pictures
IPMAR_DRICUi009A_Morphology.pdf
Morphology of DRICUi009A on day 2 post thaw
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
C-X-C Chemokine Receptor Type 4
Yes
SRY-box transcription factor 17
Yes
Morphology
IPMAR_DRICUi009A_Endoderm .pdf
IPMAR_DRICUi009A_Endoderm
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
C-X-C Chemokine Receptor Type 4
Yes
BRACHYURY
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
Paired box 6
Yes
orthodenticle homeobox 2
Yes
Morphology
IPMAR_DRICUi009A_Ectoderm.pdf
IPMAR_DRICUi009A_Ectoderm

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 13

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No gross abnormalities
Passage number: 13
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)