Edi 2

General#

Cell Line

hPSCreg Name EDe009-A
Alternative name(s)
Edi 2
Cell line type Human embryonic stem cell (hESC)
Last update 9th January 2018
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Provider

Generator University of Edinburgh (ED)
Owner College of Medicine and Veterinary Medicine
Distributors
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A234
BioSamples SAMEA104628038

General Information

Projects
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA104628037

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? No
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HEFA
Approval number R0132

hESC Derivation#

Date of derivation 2006-05-15
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Feeder cells Mouse embryo fibroblast
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: %

Characterisation#

No characterisation data could be found.

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX

Other Genotyping (Cell Line)