General

Cell Line
hPSCreg name	EDe009-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	EDe009-A (RRID:CVCL_A234)
Alternative name(s)	Edi 2
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	4th September 2022
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Provider
Generator	University of Edinburgh (ED) Contact: College of Medicine and Veterinary Medicine
Owner	College of Medicine and Veterinary Medicine
Distributors	National Institute for Biological Standards and Control - UK Stem Cell Bank (USCB)
Derivation country	United Kingdom
External Databases
Cellosaurus	CVCL_A234
BioSamples	SAMEA104628038
Wikidata	Q54831968
General Information
Projects	CerebralHominoids: Evolutionary biology of human and great ape brain development in cerebral organoids
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases
External Databases (Donor)
BioSamples	SAMEA104628037

Ethics

Was the embryo established purely for research purposes?	No
Have both parents consented to the use of the embryo for ESC derivation?	No
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent expressly prevent the derivation of pluripotent stem cells?	No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	HEFA
Approval number	R0132

hESC Derivation

Date of derivation	2006-05-15
Supernumerary embryos from IVF treatment?	Yes Separation of research and IVF treatment? Yes
PGD Embryo?	No

Culture Conditions

Feeder cells	Mouse embryo fibroblast Cellfinder Ont Id: EFO_0004040
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Knock-out serum replacement Serum concentration: %

Characterisation

No characterisation data could be found.

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XX
Other Genotyping (Cell Line)