General

Cell Line
hPSCreg name	ESe026-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	ESe026-A (RRID:CVCL_C768)
Alternative name(s)	ES-11EM
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	28th July 2017
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Provider
Generator	Spanish Stem Cell Bank (ES) Contact: Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
Derivation country	Spain
External Databases
Cellosaurus	CVCL_C768
Wikidata	Q54832750
General Information
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Hereditary multiple exostoses The donor is affected. Synonyms Multiple congenital exostosis Multiple exostosis syndromes Osteochondromatosis syndrome hereditary multiple exostoses 1 hereditary multiple exostoses 2 hereditary multiple exostoses 3 multiple ostechondromas show more synonyms show less synonyms
Disease associated phenotypes	Inherent

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Ethics Board of the Center of Regenerative Medicine in Barcelona
Approval number

hESC Derivation

Date of derivation	2009-04-24
Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	Yes
Expansion status	1 Stephenson 2007
ZP removal technique	Enzymatic
Cell isolation	None
Cell seeding	Embryo

Culture Conditions

Feeder cells	Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419
Passage method	Mechanically
O2 Concentration	20 %
CO2 Concentration	5 %
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit L-Glutamine bFGF

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
NANOG	Yes
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
SOX2	Yes
TDGF1	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vivo teratoma

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vivo teratoma

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vivo teratoma

In vitro spontaneous differentiation

Microbiology / Virus Screening
HIV 1	Negative
HIV 2	Negative
Hepatitis B	Negative
Hepatitis C	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XY Passage number: 11
Other Genotyping (Cell Line)