ES-11EM

General#

Cell Line

hPSCreg Name ESe026-A
Alternative name(s)
ES-11EM
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Spanish Stem Cell Bank (ES)
Derivation country Spain

External Databases

Cellosaurus CVCL_C768

General Information

Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
hereditary multiple exostoses
The donor is affected.
Synonyms
  • Multiple exostosis syndromes
  • Multiple congenital exostosis
  • multiple ostechondromas
  • hereditary multiple exostoses 3
  • hereditary multiple exostoses 2
  • hereditary multiple exostoses 1
  • Osteochondromatosis syndrome (disorder) [Ambiguous]
show more synonyms
Disease associated phenotypes
  • Inherent

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2009-04-24
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 1
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation None
Cell seeding Embryo

Culture Conditions#

Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SOX2
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)