pES12

General#

Cell Line

hPSCreg Name ESe027-A
Alternative name(s)
pES12
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Spanish Stem Cell Bank (ES)
Derivation country Spain

External Databases

Cellosaurus CVCL_C101

General Information

Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2010-02-03
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
No
Other hESC source: Parthogenesis
Expansion status 2
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology c
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation None
Cell seeding Embryo

Culture Conditions#

Surface coating Gelatin
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
glutamax
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)