[UCiPS] Ctrl5-R4F-25
ESi046-A
General#
Cell Line |
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hPSCreg Name | ESi046-A |
Alternative name(s) |
[UCiPS] Ctrl5-R4F-25
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator | Spanish Stem Cell Bank (ES) |
Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
Distributors | |
Derivation country | Spain |
External Databases |
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BioSamples | SAMEA104130550 |
Cellosaurus | CVCL_VD45 |
General Information |
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Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Disease associated phenotypes | no phenotypes |
Family history | no |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA104130551 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell origin |
urineExcretion that is the output of a kidney
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Age of donor (at collection) | 55-59 |
Collected in | 2016 |
Passage number reprogrammed | 2 |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
No |
Reprogramming vectors silenced? |
Yes |
Methods used |
PCR, RT-PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Other |
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Selection criteria for clones | good morphology and integration and silencing of reprogramming vectors |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium | mTeSR™ 1 |
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Unkown |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SOX2 |
Yes |
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SSEA-4 |
Yes |
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Alkaline Phosphatase |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46, XX
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Other Genotyping (Cell Line) |
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