STGD59-MiPS4F11
The cell line is not validated yet.
ESi191-A
General
iPSC Line |
|
| hPSCreg name | ESi191-A |
| Cite as: | ESi191-A |
| Alternative name(s) |
STGD59-MiPS4F11
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 28th July 2026 |
| User feedback | |
Provider |
|
| Generator |
Spanish Stem Cell Bank (ES)
Contact:
Spanish Stem Cell Bank (ES) |
| Owner | Spanish Stem Cell Bank (ES) |
External iPSC Databases |
|
| BioSamples | SAMEA123189815 |
General iPSC Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Research projects in eye diseases
Research use: allowed
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Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA123189816 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| * Does consent pertain to a specific research project? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | COMITÉ COORDINADOR DE ÉTICA DE LA INVESTIGACIÓN BIOMÉDICA DE ANDALUC |
| Approval number | 1494- N-22 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
iPSC Derivation
General |
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| Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
|
| Source cell origin |
1: The fluid that circulates in the heart, arteries, capillaries, and veins of a vertebrate animal carrying nourishment and oxygen to and bringing away waste products from all parts of the body. 2: A comparable fluid of an invertebrate.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Notes on reprogramming vector detection | No viral genes detected at pasage 8 |
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Dispase
|
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| Alpha-fetoprotein |
Yes |
Protocol or reference
AFP stgd59.pdf
AFP positive IF
In vitro spontaneous differentiation
| Marker | Expressed |
| Actin Smooth muscle |
Yes |
Protocol or reference
sma stgd59.pdf
SMA positive IF
In vitro spontaneous differentiation
| Marker | Expressed |
| TUBB3 |
Yes |
Protocol or reference
tuj1 stgd59.pdf
tuj1 positive IF
Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
|
Other Genotyping (iPSC Line) |
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