FMCPGHi002-A

301-iPSC-normal-002B

General

Cell Line

hPSCreg name FMCPGHi002-A
Cite as:
FMCPGHi002-A (RRID:CVCL_D0F0)
Alternative name(s)
301-iPSC-normal-002B
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 22nd December 2022
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Provider

Generator The First Medical Center of PLA General Hospital (FMCPGH)
Owner The First Medical Center of PLA General Hospital (FMCPGH)
Distributors
Derivation country China

External Databases

BioSamples SAMEA112262703
Cellosaurus CVCL_D0F0
Wikidata Q123031295

General Information

* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Rsearch
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Additional restrictions:

No

Donor Information

General Donor Information

Sex male
Ethnicity Chinese Han

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history No
Is the medical history available upon request? Yes
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA112262704

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Has the donor agreed to be re-contacted? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the medical research ethics committee of the PLA General Hospital
Approval number S2021-443
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-4
Yes
OCT4
Yes
NANOG
Yes
SOX2
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Morphology
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Morphology
Retinal Pigment Epithelium
Ont Id: BTO_0001177
In vivo teratoma
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,xy
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)