iCas9-NKX2-1 EGFP
The cell line is not validated yet.
GZNLi001-A-1
General
iPSC Line |
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| hPSCreg name | GZNLi001-A-1 |
| Cite as: | GZNLi001-A-1 |
| Alternative name(s) |
iCas9-NKX2-1 EGFP
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| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 18th June 2026 |
| User feedback | |
Provider |
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| Generator | Guangzhou Laboratory (GZNL) |
| Owner | Guangzhou Laboratory (GZNL) |
External iPSC Databases |
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| BioSamples | SAMEA122892933 |
General iPSC Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
iPSC Genetic Modification
| Genetic modifications not related to a disease |
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Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Ethnicity asian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA122891895 |
Ethics
Also have a look at the ethics information for the parental line
GZNLi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Addgene |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
iPSC Derivation
General |
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The source cell information can be found in the parental cell line
GZNLi001-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Selection criteria for clones | Clones were selected based on compact, dome-shaped morphology with a high nuclear-to-cytoplasmic ratio, and confirmed by positive alkaline phosphatase (AP) staining. |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
Morphology pictures
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Data analysis report
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| FOXA2 |
Yes |
| SOX17 |
Yes |
Protocol or reference
In vitro directed differentiation
| Marker | Expressed |
| HAND1 |
Yes |
| TBXT |
Yes |
Protocol or reference
In vitro directed differentiation
| Marker | Expressed |
| PAX6 |
Yes |
| OTX2 |
Yes |
Protocol or reference
Microbiology / Virus Screening |
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Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
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Other Genotyping (iPSC Line) |
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