General

iPSC Line
hPSCreg name	ICHi005-A-2
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	ICHi005-A-2
Alternative name(s)	RYR2_G1885E-HET-CORR
iPSC line type	Human induced pluripotent stem cell (hiPSC)
Similar iPSC lines	No similar lines found.
Last update	10th September 2026
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Provider
Generator	University Medical Center Hamburg-Eppendorf (UKE) Contact: University Medical Center Hamburg-Eppendorf (UKE)
External iPSC Databases
BioSamples	SAMEA123447811
General iPSC Information
* Is the cell line readily obtainable for third parties?	No
Subclone of	ICHi005-A

iPSC Genetic Modification

Disease/phenotype related modifications

Left ventricular noncompaction Genetic modifications Type of Modification Isogenic modification Chromosome location 1q43 Nucleotide sequence variant in HGVS format NM_001035.3(RYR2):c.5654G>A Protein sequence variant in HGVS format NP_001026.2:p.(Gly1885Glu) Is the modification homozygous or heterozygous? Heterozygous How has the target locus been modified? Repaired

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Left ventricular noncompaction Synonyms LVNC Lv non-compaction syndrome left ventricular hypertrabeculation left ventricular non-compaction cardiomyopathy left ventricular non-compaction syndrome left ventricular noncompaction (disease) spongy myocardium show more synonyms show less synonyms
Family history	yes
External Databases (Donor)
BioSamples	SAMEA122178167

Ethics

Also have a look at the ethics information for the parental line ICHi005-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

iPSC Derivation

General
The source cell information can be found in the parental cell line ICHi005-A.
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Is reprogramming vector detectable?	No
Methods used	PCR
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

iPSC Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzymatically Accutase
O2 Concentration	21 %
CO2 Concentration	5 %
Medium	Other medium: Base medium: DMEM/F12 Main protein source: HSA 0.1% Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line?	Yes

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
SSEA-3	Yes			–
NANOG	Yes		–
SOX2	Yes		–

Morphology

Brightfield.jpg

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Protocol or reference

FOXA2.png

SOX17.png

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Protocol or reference

Brachyury T.png

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Protocol or reference

PAX6.png

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?	Yes 46,XY Passage number: 21 Karyotyping method: NanoString
Other Genotyping (iPSC Line)