DBA 15J #3

General

Cell Line

hPSCreg name IMBAi014-A
Cite as:
IMBAi014-A
Alternative name(s)
DBA 15J #3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
IMBAi013-A
(DBA 14J #2)
Donor diseases:
Diamond-Blackfan anemia 1
IMBAi015-A
(DBA 16J #1)
Donor diseases:
Diamond-Blackfan anemia 1
IMBAi012-A
(DBA 13j #1)
Last update 22nd February 2024
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Provider

Generator Institute of Molecular Biotechnology (IMBA)
Derivation country Austria

External Databases

BioSamples SAMEA115331272

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • DBA
  • Diamond-Blackfan Anaemia type 1
  • Diamond-Blackfan Anemia type 1
  • Diamond-Blackfan anaemia caused by mutation in RPS19
  • Diamond-Blackfan anemia 1
  • Diamond-Blackfan anemia caused by mutation in RPS19
  • RPS19 Diamond-Blackfan anaemia
  • RPS19 Diamond-Blackfan anemia
  • Aase syndrome
  • Aase-Smith syndrome 2
  • Blackfan-Diamond syndrome
  • DBA1
  • Red cell aplasia, Pure, hereditary
  • anemia, congenital erythroid hypoplastic
  • anemia, congenital hypoplastic, of Blackfan and Diamond
  • aregenerative Anemia, chronic congenital
  • erythrogenesis imperfecta
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
X, Y
Karyotyping method: Molecular karyotyping by SNP array
http://

Donor Relations

All cell lines of this donor's relatives
Has mother:
Has father:
Has sister:

External Databases (Donor)

BioSamples SAMEA115331289

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information leo.kager@stanna.at
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethikkommission der Medizinischen Universität Wien
Approval number 1994/2016
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Medium Other medium:
Base medium: StemFlex™ Medium
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CD140b
Yes
CDH5
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
SOX2
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
X,Y
Passage number: 17
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)