IV-B hiPSC line, DESY-HE
The cell line is not validated yet.
INEUi007-A
General
Cell Line |
|
| hPSCreg name | INEUi007-A |
| Cite as: | INEUi007-A |
| Alternative name(s) |
IV-B hiPSC line, DESY-HE
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 16th March 2026 |
| User feedback | |
Provider |
|
| Generator | Instituto de Neurociencias Conicet (INEU) |
| Owner | Instituto de Neurociencias Conicet (INEU) |
| Distributors | |
| Derivation country | Argentina |
External Databases |
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| BioSamples | SAMEA121971887 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Lebanese |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
| Disease associated phenotypes |
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| Family history | https://doi.org/10.1136/jmg-2025-110852 |
| Is the medical history available upon request? | Yes |
| Is clinical information available? | Yes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| All cell lines of this donor's relatives |
Has sister:
Has brother:
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External Databases (Donor) |
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| BioSamples | SAMEA121971888 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| * Does consent pertain to a specific research project? | No |
| Does consent permit unforeseen future research, without further consent? | No |
| Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Mononuclear cells collected from peripheral blood.
Synonyms
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Yes |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: Gibco StemFlex Medium
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| Alkaline Phosphatase |
Yes |
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| CD326 |
Yes |
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| TUBB3 |
Yes |
| NES |
Yes |
Morphology
Immunofluorescence corresponding to INEUi007-A is 'EBs derivados de hiPSCs DESY-HE' column and 'NES' and 'TUBB3' rows.
RT-qPCR corresponding to INEUi007-A is the yellow colour.
In vitro spontaneous differentiation
| Marker | Expressed |
| NKX2-5 |
Yes |
| DES |
Yes |
Morphology
RT-qPCR corresponding to INEUi007-A is the yellow colour.
Immunofluorescence corresponding to INEUi007-A is 'EBs derivados de hiPSCs DESY-HE' column and 'DES' row.
In vitro spontaneous differentiation
| Marker | Expressed |
| AFP |
Yes |
| SOX17 |
Yes |
Morphology
Immunofluorescence corresponding to INEUi007-A is 'EBs derivados de hiPSCs DESY-HE' column and 'AFP' and 'SOX17' rows.
RT-qPCR corresponding to INEUi007-A is the yellow colour.
RT-qPCR corresponding to INEUi007-A is the yellow colour.
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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