OSCAR
INSRMe001-A
General
Cell Line |
|
| hPSCreg name | INSRMe001-A |
| Cite as: | INSRMe001-A (RRID:CVCL_B020) |
| Alternative name(s) |
OSCAR
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 3rd September 2022 |
| User feedback | |
Provider |
|
| Generator |
INSERM (INSRM)
Contact:
PrimaStem |
| Owner | PrimaStem |
| Distributors | |
| Derivation country | France |
External Databases |
|
| Cellosaurus | CVCL_B020 |
| Wikidata | Q54936694 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
| Date of derivation | 2008-01-08 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
1
|
| ICM morphology |
C
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Enzymatic |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
| Derived under xeno-free conditions? |
No |
| Derivation under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Gelatin | |||||||||
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method |
Enzymatically
Collagenase
|
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| O2 Concentration | 5 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
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| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| GCTM2 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
|
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 37
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
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