IRFMNi003-A-6

KO PKD1#37

The cell line is not validated yet.

General

iPSC Line

hPSCreg name IRFMNi003-A-6
Cite as:
IRFMNi003-A-6
Alternative name(s)
KO PKD1#37
iPSC line type Human induced pluripotent stem cell (hiPSC)
Similar iPSC lines No similar lines found.
Last update 4th September 2026
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Provider

Generator IRCCS - Istituto di Ricerche Farmacologiche Mario Negri (IRFMN)

External iPSC Databases

BioSamples SAMEA123169238

General iPSC Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

iPSC Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Polycystic kidneys - adult type
  • Autosomal dominant adult polycystic kidney disease
  • ADPKD
  • adult type autosomal dominant polycystic kidney disease
  • Polycystic kidney disease, adult type (disorder)
  • polycystic kidney disease, adult type
  • ADPKD - Autosomal dominant polycystic kidney disease
  • polycystic kidney, autosomal dominant
  • Autosomal dominant polycystic kidney disease
show more synonyms
Genetic modifications
PKD1 (target)
Gene knock-out
16p13.3
CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5843913

Ethics

Also have a look at the ethics information for the parental line IRFMNi003-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

iPSC Derivation

General

The source cell information can be found in the parental cell line IRFMNi003-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

iPSC Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
Medium mTeSR™ 1

iPSC Characterisation

Analysis of undifferentiated iPSCs
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
alpha fetoprotein
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
smooth muscle actin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
tubulin beta 3 class III
Unknown

Genotyping

Karyotyping (iPSC Line)

Has the iPSC line karyotype been analysed?
Yes
46,XX
Passage number: 29

Other Genotyping (iPSC Line)