4603c53_LC3
ISTEMi015-A-1
General
Cell Line |
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| hPSCreg name | ISTEMi015-A-1 |
| Cite as: | ISTEMi015-A-1 |
| Alternative name(s) |
4603c53_LC3
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 2nd February 2026 |
| User feedback | |
Provider |
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| Generator | Institut from Stem cell Therapy and Exploration of Monogenic diseases (ISTEM) |
| Owner | Institut from Stem cell Therapy and Exploration of Monogenic diseases (ISTEM) |
| Distributors | |
| Derivation country | France |
External Databases |
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| BioSamples | SAMEA120561741 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | Clinically unaffected; father of two affected daughters (GM04601, GM04602); affected spouse is GM04608; see GM04604 lymphocyte |
| Is clinical information available? | Clinically unaffected; father of two affected daughters (GM04601, GM04602); affected spouse is GM04608; see GM04604 lymphocyte |
External Databases (Donor) |
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| BioSamples | SAMEA120558811 |
Ethics
Also have a look at the ethics information for the parental line
ISTEMi015-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | For integration into the AAVS1 gene, a home-made plasmid was used; for the HiBiT part of the complete plasmid, Promega products were used |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
ISTEMi015-A.
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Reprogramming method |
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Vitronectin | ||||||
| Feeder cells |
No |
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| Passage method | Mechanically | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: StemMACS™ iPS-Brew Basal Medium XF, human
Main protein source: Serum concentration: % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| NANOG |
Yes |
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
Morphology
In vitro spontaneous differentiation
Morphology
In vitro spontaneous differentiation
Morphology
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
no mutation |
Genetic Modification
| Genetic modifications not related to a disease |
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