JNCSRe001-A-1

BJNhem19-TBXT-2A-EGFP

General

iPSC Line

hPSCreg name JNCSRe001-A-1
Cite as:
JNCSRe001-A-1
Alternative name(s)
BJNhem19-TBXT-2A-EGFP
iPSC line type Human embryonic stem cell (hESC)
Similar iPSC lines No similar lines found.
Last update 12th May 2026
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Provider

Generator Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Owner Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR)
Distributors
Derivation country India

External iPSC Databases

BioSamples SAMEA122228492

General iPSC Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

MTA required.

Subclone of

iPSC Genetic Modification

Genetic modifications not related to a disease
TBXT (human) (target)
Gene knock-in
6q27
EGFP
6q27
Targeting at the C-terminus of Endogenous Brachyury/TBXT gene to generate the EGFP reporter line.
CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information

Sex male
Ethnicity Asian Indian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA122409947

Ethics

Also have a look at the ethics information for the parental line JNCSRe001-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution Research use only. Can be distributed only after required approvals and MTA.

hESC Derivation

The source cell information can be found in the parental cell line JNCSRe001-A.

iPSC Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
Yes
Passage method Mechanically
O2 Concentration 0 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line?
Yes

iPSC Characterisation

Analysis of undifferentiated iPSCs
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
Morphology pictures
BJNhem19_TBXT_2A_EGFP_colony
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
Alpha fetoprotein
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
TBXT/ Brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Class III beta tubulin
Yes

Genotyping

Karyotyping (iPSC Line)

Has the iPSC line karyotype been analysed?
Yes
46,XY (Normal Karyotye)
Passage number: 6
Karyotyping method: G-Banding

Other Genotyping (iPSC Line)