BJNhem19-TBXT-2A-EGFP
JNCSRe001-A-1
General
Cell Line |
|
| hPSCreg name | JNCSRe001-A-1 |
| Cite as: | JNCSRe001-A-1 |
| Alternative name(s) |
BJNhem19-TBXT-2A-EGFP
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 12th May 2026 |
| User feedback | |
Provider |
|
| Generator | Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR) |
| Owner | Jawaharlal Nehru Centre for Advanced Scientific Research (JNCSR) |
| Distributors | |
| Derivation country | India |
External Databases |
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| BioSamples | SAMEA122228492 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
MTA required. |
| Subclone of | |
Genetic Modification
| Genetic modifications not related to a disease |
|
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Asian Indian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA122409947 |
Ethics
Also have a look at the ethics information for the parental line
JNCSRe001-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | Research use only. Can be distributed only after required approvals and MTA. |
hESC Derivation
|
The source cell information can be found in the parental cell line
JNCSRe001-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
Yes |
| Passage method | Mechanically |
| O2 Concentration | 0 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| SSEA-4 |
Yes |
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Morphology pictures
BJNhem19_TBXT_2A_EGFP_colony
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XY (Normal Karyotye)
Passage number: 6
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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