General

iPSC Line
hPSCreg name	KAIMRCi007-B
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	KAIMRCi007-B
Alternative name(s)	AOA2-Hom-iPSC#2
iPSC line type	Human induced pluripotent stem cell (hiPSC)
Similar iPSC lines	No similar lines found.
Last update	17th July 2026
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Provider
Generator	King Abdullah International Medical Research Center (KAIMRC)
General iPSC Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: allowed

Donor Information

General Donor Information
Sex	male
Ethnicity	Saudi
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Spinocerebellar Ataxia, Autosomal Recessive, with Axonal Neuropathy 2 The donor is a carrier of a disease-associated mutation and affected. Synonyms AOA2 Ataxia with Oculomotor Apraxia Type 2 SCAN2 Spinocerebellar Ataxia, Autosomal Recessive, with Axonal Neuropathy 2 show more synonyms show less synonyms Genetic variants SETX (target) Chromosome location 9q34.13 Nucleotide sequence variant in HGVS format c.2143_2144delGT Protein sequence variant in HGVS format p.Val715Lysfs*22 Is the variant homozygous or heterozygous? Homozygous
Is clinical information available?	yes
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	Yes
Donor Relations
Other cell lines of this donor	KAIMRCi007-A
External Databases (Donor)
BioSamples	SAMEA123134085

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
* Does consent expressly prevent the derivation of pluripotent stem cells?	No
* Does consent pertain to a specific research project?	Yes
Details on restriction to research project	This line is part of the Saudi Bank of iPSCs
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
How may genetic information associated with the cell line be accessed?	Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	King Abdullah International Medical Research Center
Approval number	NRJ22J/060/03
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

iPSC Derivation

General
Source cell line name	erythroid progenitor cells (EPCs) Derived from same source line (potentially other lot and donor, see below): EMCi229-A KAIMRCi003-A KAIMRCi003-B KAIMRCi004-A KAIMRCi004-B KAIMRCi005-A KAIMRCi007-A KAIMRCi010-A KAIMRCi010-B KAIMRCi011-A KAIMRCi011-B KAIMRCi019-A KAIMRCi019-B
Source cell type	Erythroblast An immature, nucleated erythrocyte occupying the stage of erythropoiesis that follows formation of erythroid progenitor cells and precedes formation of reticulocytes. Synonyms Nucleated RBC Nucleated Red Blood Cells Nucleated Red Cell Nucleated Erythrocyte Nucleated Red Blood Cell Erythroblasts Nucleated Erythrocytes Nucleated Red Cells Erythroblast show more synonyms show less synonyms
Source cell origin	Whole Blood Blood that has not been separated into its various components; blood that has not been modified except for the addition of an anticoagulant. Synonyms WHOLE BLOOD Whole Blood Peripheral Whole Blood Whole blood show more synonyms show less synonyms
Passage number reprogrammed	1
Reprogramming method
Vector type	Non-integrating
Vector	Episomal
Genes	POU5F1 SOX2 KLF4 MYC LIN28
Is reprogramming vector detectable?	No
Methods used	PCR
Files and images showing reprogramming vector expressed or silenced	EPi5_PCR.tif
Vector free reprogramming
Other
Selection criteria for clones	Based on hESC-like morphology, emerging iPSCs were and picked and expanded into feeder-free culture conditions.
Derived under xeno-free conditions	Yes
Derived under GMP?	No
Available as clinical grade?	No

iPSC Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzyme-free cell dissociation Gentle Cell Dissociation Reagent
O2 Concentration	20 %
CO2 Concentration	5 %
Medium	mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line?	Yes

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
POU5F1 (OCT-4)	Yes
NANOG	Yes
SOX2	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

Marker	Expressed
SOX17	Yes
GATA4	Yes

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

Marker	Expressed
Brachyury or TBXT	Yes
DES	Yes
CDX2	Yes

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

Marker	Expressed
PAX6	Yes
NESTIN or NES	Yes

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?	Yes 46,XY Passage number: 17 Karyotyping method: G-Banding
Other Genotyping (iPSC Line)
Is there genome-wide genotyping or functional data available?	Yes