General

Cell Line
hPSCreg name	KCLe015-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	KCLe015-A (RRID:CVCL_A245)
Alternative name(s)	KCL011
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	28th July 2017
User feedback show/hide	Written by on Delete No feedback available yet. Login to share your feedback, experiences or results with the research community.
Provider
Generator	King's College London (KCL) Contact: Department of Women's Health
Derivation country	United Kingdom
External Databases
Cellosaurus	CVCL_A245
NIHhESC	NIHhESC-13-0212
Wikidata	Q54899659
General Information
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number	NIHhESC-13-0212

hESC Derivation

Date of derivation	2009-06-11
Embryo stage	Blastula with ICM and Trophoblast
Expansion status	5 Stephenson 2007
ICM morphology	C Stephenson 2007
Trophectoderm morphology	b Stephenson 2007
ZP removal technique	Mechanical
Cell isolation	None
Cell seeding	Embryo

Culture Conditions

Surface coating	Cellstart
Feeder cells	Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419
O2 Concentration	5 %
CO2 Concentration	6 %
Medium	Other medium: Base medium: KOSR-XF Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements Supplement Amount Unit FGF 2

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
NANOG	Yes
POU5F1 (OCT-4)	Yes
TRA 1-60	Yes
TRA 1-81	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XX Passage number: 7
Other Genotyping (Cell Line)