KCL011

General

Cell Line

hPSCreg Name
KCLe015-A
Alternative name(s)
KCL011
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A245
NIHhESC NIHhESC-13-0212
CLO CLO_0100526
Wikidata Q54899659

General Information

* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-13-0212

hESC Derivation

Date of derivation 2009-06-11
Embryo stage Blastula with ICM and Trophoblast
Expansion status 5
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Mechanical
Cell isolation None
Cell seeding Embryo

Culture Conditions

Surface coating Cellstart
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 5 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: KOSR-XF
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
FGF 2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 7

Other Genotyping (Cell Line)