KIe011-A-1
General
Cell Line |
|
| hPSCreg name | KIe011-A-1 |
| Cite as: | KIe011-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
KIe011-A (HS362) |
| Last update | 11th April 2019 |
| User feedback | |
Provider |
|
| Generator |
Karolinska Institutet (KI)
Contact:
Stem Cell Laboratory |
| Derivation country | Sweden |
External Databases |
|
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
KIe011-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
|
The source cell information can be found in the parental cell line
KIe011-A.
|
Culture Conditions
| Feeder cells |
Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
||||||
| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
|
|||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| TDGF1 |
Yes |
|
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Passage number: 41
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.