KIe022-A-1
General
Cell Line |
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| hPSCreg name | KIe022-A-1 |
| Cite as: | KIe022-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
KIe022-A (HS420) |
| Last update | 11th April 2019 |
| User feedback | |
Provider |
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| Generator |
Karolinska Institutet (KI)
Contact:
Stem Cell Laboratory |
| Derivation country | Sweden |
External Databases |
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General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
KIe022-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
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The source cell information can be found in the parental cell line
KIe022-A.
|
Culture Conditions
| Feeder cells |
Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
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| O2 Concentration | 21 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-1 |
No |
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Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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