General

iPSC Line
hPSCreg name	KIe033-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	KIe033-A-1
iPSC line type	Human embryonic stem cell (hESC)
Similar iPSC lines	KIe033-A (HS980)
Last update	14th June 2024
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Provider
Generator	Karolinska Institutet (KI)
External iPSC Databases
BioSamples	SAMEA115738885
General iPSC Information
* Is the cell line readily obtainable for third parties?	No
Subclone of	KIe033-A

iPSC Genetic Modification

Genetic modifications not related to a disease

msx1 (target) Type of iPSC Modification Transgene expression Chromosome location 4p16.2 Free text Stop codon of MSX1 was removed and replaced with T2A-tdTomato-EF1a-BSD. Insertion validated with PCR, sequencing and expression of transgene. Delivery method CRISPR-associated (CRISPR/Cas) System ALCAM (target) Type of iPSC Modification Transgene expression Chromosome location 3q13.11 Free text Stop codon of ALCAM was removed and replaced with T2A-EGFP-EF1a-Puro. Insertion validated with PCR, sequencing and expression of transgene. Delivery method CRISPR-associated (CRISPR/Cas) System NTN1 (target) Type of iPSC Modification Transgene expression Chromosome location 17p13.1 Free text Stop codon of NTN1 was removed and replaced with T2A-mTagBFP2-EF1a-Neo. Insertion validated with PCR, sequencing and expression of transgene. Delivery method CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
External Databases (Donor)
BioSamples	SAMEA6407484

Ethics

Also have a look at the ethics information for the parental line KIe033-A .

hESC Derivation

The source cell information can be found in the parental cell line KIe033-A.

iPSC Culture Conditions

iPSC Characterisation

No characterisation data could be found for this subclone. Please open parental cell line KIe033-A .

Genotyping

Karyotyping (iPSC Line)

Other Genotyping (iPSC Line)