hPSCreg®
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KIe033-A-1

Registration Summary:
KIe033-A-1

General

Cell Line
hPSCreg name KIe033-A-1
Cite as:
KIe033-A-1
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 14th June 2024
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Provider
Generator Karolinska Institutet (KI)

External Databases
BioSamples SAMEA115738885

General Information
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information
Sex female

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

External Databases (Donor)
BioSamples SAMEA6407484

Ethics

Also have a look at the ethics information for the parental line KIe033-A .

hESC Derivation

The source cell information can be found in the parental cell line KIe033-A.

Culture Conditions

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line KIe033-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
msx1 (target)
Transgene expression
4p16.2
Stop codon of MSX1 was removed and replaced with T2A-tdTomato-EF1a-BSD. Insertion validated with PCR, sequencing and expression of transgene.
CRISPR-associated (CRISPR/Cas) System
ALCAM (target)
Transgene expression
3q13.11
Stop codon of ALCAM was removed and replaced with T2A-EGFP-EF1a-Puro. Insertion validated with PCR, sequencing and expression of transgene.
CRISPR-associated (CRISPR/Cas) System
NTN1 (target)
Transgene expression
17p13.1
Stop codon of NTN1 was removed and replaced with T2A-mTagBFP2-EF1a-Neo. Insertion validated with PCR, sequencing and expression of transgene.
CRISPR-associated (CRISPR/Cas) System