LRB013

General#

Cell Line

hPSCreg Name KUe011-A
Alternative name(s)
LRB013
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator University of Copenhagen (KU)
Derivation country Denmark

External Databases

Cellosaurus CVCL_B247

General Information

Is the cell line available in principle?
No

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Passage method Mechanically

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)