LRB014
KUe012-A
General
Cell Line |
|
| hPSCreg name | KUe012-A |
| Cite as: | KUe012-A (RRID:CVCL_B248) |
| Alternative name(s) |
LRB014
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 3rd September 2022 |
| User feedback | |
Provider |
|
| Generator |
University of Copenhagen (KU)
Contact:
Laboratory of Reproductive Biology |
| Derivation country | Denmark |
External Databases |
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| Cellosaurus | CVCL_B248 |
| Wikidata | Q54903023 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
| Date of derivation | 2007-11-06 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
C
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Mechanical |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
Yes |
| Passage method | Mechanically |
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| TDGF1 |
Yes |
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Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
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