LUMCi019-A-1

isoLUMC0110iALK

General#

Cell Line

hPSCreg Name LUMCi019-A-1
Alternative name(s)
isoLUMC0110iALK
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th February 2020
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Provider

Generator Leiden University Medical Center (LUMC)

External Databases

BioSamples SAMEA6712312

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hereditary Hemorrhagic Telangiectasia
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Telangiectasia, Hereditary Hemorrahagic, of Rendu, Osler
  • Osler-Weber-Rendu Disease
  • Hereditary Hemorrhagic Telangiectasia

External Databases (Donor)

BioSamples SAMEA6712311

Ethics#

Also have a look at the ethics information for the parental line LUMCi019-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line LUMCi019-A.
Passage number reprogrammed 3

Reprogramming method

Vector type Integrating
Vector Plasmid
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
NCAM
Yes
Vimentin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
TUBB3
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: Array CGH

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Hereditary Hemorrhagic Telangiectasia
Synonyms
  • Telangiectasia, Hereditary Hemorrahagic, of Rendu, Osler
  • Osler-Weber-Rendu Disease
  • Hereditary Hemorrhagic Telangiectasia
Genetic modifications
ACVRL1 (target)
Isogenic modification
Heterozygous
Repaired