LUMC0322iFOPA-03

The cell line is not validated yet.

General

Cell Line

hPSCreg name LUMCi062-A
Cite as:
LUMCi062-A
Alternative name(s)
LUMC0322iFOPA-03
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 31st July 2025
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Provider

Generator Leiden University Medical Center (LUMC)
Owner Leiden University Medical Center (LUMC)
Distributors
Derivation country Netherlands

External Databases

BioSamples SAMEA117637321

General Information

* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Research related to single ventricle disease or congenital heart disease related to the right ventricle
Research use: allowed
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Donor was born with tricuspid atresia, a hypoplastic right ventricle, pulmonary stenosis, a persisting left superior vena cava, and a persisting left inferior vena cava draining in the left renal vein. Following Fontan palliation, the donor developed protein-losing enteropathy. The donor also developed short self-terminating episodes of atrial tachycardias/flutter or non-sustained ventricular tachycardia.
The donor is affected.
Stage
Post-operative
Synonyms
  • Single Ventricle
  • Single Ventricle Defect
Genetic variants
The donor harbours a missense variant of unknown significance in the BRAF gene, which is related to RASopathies. Although cardiofaciocutaneous syndrome cannot yet be formally diagnosed in the absence of a (likely) pathogenic BRAF variant classification, the donor shows several features of cardiofaciocutaneous syndrome. These features include high myopia, a horseshoe kidney, sacral hypoplasia, right hip dysplasia, and several dysmorphic physical features. However, the donor shows high intellectual performance, which is not typical of cardiofaciocutaneous syndrome.
Synonyms
  • Birth Defect
  • CONGENITAL DEFECT/DEFORMITY
  • Congenital Abnormality
  • Congenital Anatomic Abnormality
  • Congenital Anatomical Abnormality
  • Congenital Anomalies of Fetus
  • Congenital Anomaly
  • Congenital Anomaly or Birth Defect
  • Congenital Defect
  • Congenital Defect/Deformity
  • Congenital Deformity
  • Congenital Malformation
  • DEFECT/DEFORMITY, CONGENITAL
  • SCONG
show more synonyms
Genetic variants
BRAF (target)
7q34
NM_004333.6:c.1897T > C
NP_004324.2:p.(Tyr633His)
Heterozygous
VCV001319116.7
PMID: 37123657
Is the medical history available upon request? Medical history can be found in the following case reports: https://doi.org/10.1093/ehjcr/ytad176, https://doi.org/10.1093/ehjcr/ytac067.
Is clinical information available? Subject to approval from a medical ethics commitee, TNO-AZL Adult Quality of Life data is additionally available.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA117637535

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
* Does consent expressly prevent the derivation of pluripotent stem cells? No
* Does consent pertain to a specific research project? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: Via a request to and assessment by an independent review committee
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? LUMC Biobank Review Committee
Approval number BB23.005
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
Mononuclear cells collected from peripheral blood.
Synonyms
  • Peripheral Blood Mononuclear cells
  • Derived Peripheral Blood Mononuclear Cell
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
Morphology pictures
40X magnification phase contrast
100X magnification phase contrast
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
GATA4
Yes
Morphology
FOXA2
GATA4
SOX17
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT (Brachyury)
Yes
CDX2
Yes
VIM (Vimentin)
Yes
Morphology
TBXT (Brachyury)
CDX2
VIM (Vimentin)
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Nestin
Yes
TUBB3
Yes
PAX6
Yes
Morphology
Nestin
TUBB3
PAX6

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY, Normal
Passage number: 4
Karyotyping method: Digital PCR (iCS-digital™ PSC)

Other Genotyping (Cell Line)