VS-4F, LVIP01-LC12-1
The cell line is not submitted yet.
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General
Cell Line |
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| hPSCreg name | LVPEIi006-C |
| Cite as: | LVPEIi006-C |
| Alternative name(s) |
VS-4F, LVIP01-LC12-1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 18th September 2025 |
| Notes | This is a LCA12 patient-specific, induced pluripotent stem cell line, carrying a homozygous mutation in RD3 gene, at the exon 2 splice donor site. This mutation (c.296+1 G>A) results in splice skipping and inclusion of Intron 2, frameshift, and premature translational termination after codon 99. This iPSC line was generated by the reprogramming of donor skin-derived human dermal fibroblasts (HDFs) using retroviral vectors encoding hOCT3/4, hSOX2, hKLF4 and hcMYC transgenes (Addgene Plasmids #17217, #17218, #17219, #17220 respectively). This iPSC line maintained proper colony morphology, expressed the stemness and pluripotency markers OCT4, SOX2, NANOG, KLF4, SSEA4, and exhibited a normal karyotype at passage 20. This line efficiently differentiates into all three lineages and could generate retinal organoids and mature retinal pigmented epithelial cells. This line does not have integrated copies of the cMYC transgene in the genome. |
| User feedback | |
Provider |
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| Generator |
L.V. Prasad Eye Institute (LVPEI)
Contact:
L.V. Prasad Eye Institute (LVPEI) |
| Owner | L.V. Prasad Eye Institute (LVPEI) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA120226515 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
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hIPSC Derivation
General |
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