3E5, CL220420-16634
The cell line is not validated yet.
MILi002-B
General
Cell Line |
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| hPSCreg name | MILi002-B |
| Cite as: | MILi002-B |
| Alternative name(s) |
3E5, CL220420-16634
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 5th May 2026 |
| User feedback | |
Provider |
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| Generator |
Miltenyi Biotec B.V. & Co. KG (MIL)
Contact:
Miltenyi Biotec B.V. & Co. KG (MIL) |
| Owner | Miltenyi Biotec B.V. & Co. KG (MIL) |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA122378117 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | European, white |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA121391658 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| * Does consent pertain to a specific research project? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | French ministry of research |
| Approval number | N° AC-2023-5431 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell line name | Fibroblasts (CL220321-16713) |
| Source cell type |
Any skin fibroblast that is part of a skin of abdomen.
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| Source cell origin |
A zone of skin that is part of an abdomen [Automatically generated definition].
Synonyms
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| Source cell type (free text) | Age 61 |
| Passage number reprogrammed | 2 |
Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
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| mRNA | |
Other |
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| Selection criteria for clones | Cell sorting was performed using a closed, cartridge-based microfluidic system (positive selection of TRA-1-60+ cells) |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Laminin | ||||||
| Feeder cells |
No |
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| Passage method |
Enzymatically
TrypLE
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| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: StemMACS™ PSC-Brew XF, human
Main protein source: Serum concentration: % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-60 |
Yes |
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| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-4 |
Yes |
Morphology pictures
Differentiation Potency
In vitro directed differentiation
Morphology
In vitro directed differentiation
Morphology
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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