MMC-iPSC-002-A

The cell line is not validated yet.

General

Cell Line

hPSCreg name MMCi002-A
Cite as:
MMCi002-A (RRID:CVCL_C1US)
Alternative name(s)
MMC-iPSC-002-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
KEIUi004-A
(EYA4-1)
Donor diseases:
Hearing Loss
KEIUi006-A
(EYA4-3)
Donor diseases:
Hearing Loss
KEIUi007-A
(EYA4-4)
Donor diseases:
Hearing Loss
KEIUi005-A
(EYA4-2)
Donor diseases:
Hearing Loss
KEIUi008-A
(A1555G)
Donor diseases:
Hearing Loss
Last update 26th January 2022
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Provider

Generator Mackay Medical College (MMC)

External Databases

BioSamples SAMEA12546656
Cellosaurus CVCL_C1US
Wikidata Q114312310

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Hearing Loss
Genetic variants
GJB2 (target)
13q12.11

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA12546657

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? MacKay Memorial Hospital
Approval number 14MMIHIS249
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: Stemflex
Main protein source:
Serum concentration: %
Supplements
Gibco™ StemFlex™ 10 %

Characterisation

Analysis of Undifferentiated Cells
Marker Present Absent
mCpG
OCT4 X
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)