General

Cell Line
hPSCreg name	MNZTASi006-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	MNZTASi006-A (RRID:CVCL_D0IQ)
Alternative name(s)	MS_0005, TOB-02161, 2161-4
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	ESi123-A (PPMS PBiPS8-Sv4F-13) Donor diseases: primary progressive multiple sclerosis ESi124-A (PPMS PBiPS9-Sv4F-12) Donor diseases: primary progressive multiple sclerosis ESi122-A (PPMS PBiPS7-Sv4F-5) Donor diseases: primary progressive multiple sclerosis ESi128-A (PPMS PBiPS10-Sv4F-1) Donor diseases: primary progressive multiple sclerosis MNZTASi001-A (MS_0004, 2125-2, TOB-02125) Donor diseases: primary progressive multiple sclerosis
Last update	29th January 2024
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Provider
Generator	Menzies Institute for Medical Research (MNZTAS) Contact: Menzies Institute for Medical Research (MNZTAS)
Owner	Menzies Institute for Medical Research (MNZTAS)
Distributors	Menzies Institute for Medical Research (MNZTAS)
Derivation country	Australia
External Databases
Cellosaurus	CVCL_D0IQ
BioSamples	SAMEA114591112
Wikidata	Q123033081
General Information
* Is the cell line readily obtainable for third parties?	Yes Cell line can only be used in: research in neurodegenerative diseases or other human health conditions Research use: allowed

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Primary progressive multiple sclerosis Synonyms primary-progressive MS multiple sclerosis primary progressive PPMS primary-progressive multiple sclerosis primary progressive multiple sclerosis show more synonyms show less synonyms
Karyotyping (Donor)
Has the donor karyotype been analysed?	Unknown
External Databases (Donor)
BioSamples	SAMEA114597869

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	No
Please provide contact information of the holder of the original Donor Information Sheet.	Menzies Institute for Medical Research
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
How may genetic information associated with the cell line be accessed?	Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	University of Tasmania Human Research Ethics Committee
Approval number	H0016915
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Peripheral Blood Mononuclear Cell A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes. Synonyms PERIPHERAL BLOOD MONONUCLEAR CELL Peripheral Blood Mononuclear Cell PBMC
Source cell origin	Arm The portion of the upper extremity between the shoulder and the elbow. For clinical purposes this term is also used to refer to the whole superior limb. Synonyms ARM Arm Brachium Upper Arm show more synonyms show less synonyms
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Is reprogramming vector detectable?	No
Methods used	RT-PCR
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzymatically Dispase
O2 Concentration	20 %
CO2 Concentration	5 %
Medium	mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
OCT4	Yes		–
SOX2	Yes		–

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

Marker	Expressed
HNF4A	Yes

Morphology

EB markers MS4 and MS5.pdf

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

Marker	Expressed
GATA4	Yes

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

Marker	Expressed
Nestin	Yes

Microbiology / Virus Screening
Mycoplasma	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes female 2161-4_karyogram.pdf
Other Genotyping (Cell Line)