CA2
MSHRIe002-A
General
Cell Line |
|
| hPSCreg name | MSHRIe002-A |
| Cite as: | MSHRIe002-A (RRID:CVCL_B845) |
| Alternative name(s) |
CA2
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Mount Sinai Hospital (MSHRI)
Contact:
Nagy's Lab |
| Derivation country | Canada |
External Databases |
|
| Cellosaurus | CVCL_B845 |
| NIHhESC | NIHhESC-11-0138 |
| Wikidata | Q54906848 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent | |
| Alternative consent approval number | NIHhESC-11-0138 |
hESC Derivation
| Cell isolation | Immunosurgery |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Enzymatically | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 8 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| TDGF1 |
Yes |
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| SSEA-1 |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
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