MUNIe002-A-1

General

Cell Line

hPSCreg name MUNIe002-A-1
Cite as:
MUNIe002-A-1
Cell line type Human embryonic stem cell (hESC)
Similar lines
MUNIe002-A
(CCTL 8)
Last update 28th July 2017
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Provider

Generator Masaryk University (MUNI)
Derivation country Czech Republic

External Databases

General Information

* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Also have a look at the ethics information for the parental line MUNIe002-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line MUNIe002-A.

Culture Conditions

Feeder cells Mouse Embryonic Fibroblast Cells
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source:
Serum concentration: %
Supplements
FGF2 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47,XX,+12,del(18)(q21.2q21.3)
Passage number: 50

Other Genotyping (Cell Line)

Genetic Modification