MUNIe002-A-1
General
Cell Line |
|
| hPSCreg name | MUNIe002-A-1 |
| Cite as: | MUNIe002-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
MUNIe002-A (CCTL 8) |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator |
Masaryk University (MUNI)
Contact:
Petr Dvorak's Laboratory |
| Derivation country | Czech Republic |
External Databases |
|
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
MUNIe002-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
|
The source cell information can be found in the parental cell line
MUNIe002-A.
|
Culture Conditions
| Feeder cells |
Mouse Embryonic Fibroblast Cells Cellfinder Ont Id: EFO_0004040 |
||||||
| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Serum concentration: % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
|
|
||
| SSEA-3 |
Yes |
|
|
|||
| SSEA-4 |
Yes |
|
|
|||
| TRA 1-60 |
Yes |
|
|
|||
| TRA 1-81 |
Yes |
|
|
|||
| SSEA-1 |
No |
|
|
|||
| DNMT3B |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| GABRB3 |
Yes |
|
||||
| NANOG |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
47,XX,+12,del(18)(q21.2q21.3)
Passage number: 50
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.