MUNIe004-A-1
General
Cell Line |
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| hPSCreg name | MUNIe004-A-1 |
| Cite as: | MUNIe004-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
MUNIe004-A (CCTL 10) |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
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| Generator |
Masaryk University (MUNI)
Contact:
Petr Dvorak's Laboratory |
| Derivation country | Czech Republic |
External Databases |
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General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
MUNIe004-A
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| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
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The source cell information can be found in the parental cell line
MUNIe004-A.
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Culture Conditions
| Feeder cells |
Mouse Embryonic Fibroblast Cells Cellfinder Ont Id: EFO_0004040 |
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| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Serum concentration: % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SSEA-1 |
No |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| GABRB3 |
Yes |
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| NANOG |
Yes |
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| TDGF1 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XX,der(8)t(8;17)(p11.2;q11),del(18)(q21.2q21)[5]/46,XX,del(18)(q21.2q21.3)[10]
Passage number: 158
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Other Genotyping (Cell Line) |
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