MUNIe005-A-1

General#

Cell Line

hPSCreg Name
MUNIe005-A-1
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Masaryk University (MUNI)
Derivation country Czech Republic

External Databases

CLO CLO_0102879

General Information

* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Also have a look at the ethics information for the parental line MUNIe005-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation#

The source cell information can be found in the parental cell line MUNIe005-A.

Culture Conditions#

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source:
Serum concentration: %
Supplements
FGF2 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX der.(10)t(7,10)(p14;p12)
Passage number: P167

Other Genotyping (Cell Line)

Genetic Modification#