General

Cell Line
hPSCreg name	MUNIe014-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	MUNIe014-A
Alternative name(s)	MUES 7
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	9th June 2025
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Provider
Generator	Masaryk University (MUNI)
External Databases
BioSamples	SAMEA118448855
General Information
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	unknown
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Karyotyping (Donor)
Has the donor karyotype been analysed?	Unknown
External Databases (Donor)
BioSamples	SAMEA118448856

Ethics

Was the embryo established purely for research purposes?	No
Have both parents consented to the use of the embryo for ESC derivation?	Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	No
If you do not hold the Donor Consent Form, do you know who does?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
* Does consent expressly prevent the derivation of pluripotent stem cells?	No
* Does consent pertain to a specific research project?	No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
How may genetic information associated with the cell line be accessed?	No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?
Approval number

hESC Derivation

Date of derivation	2020-05-15
Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	Yes Separation of research and IVF treatment? Yes
PGD Embryo?	Yes

Culture Conditions

Surface coating	Laminin
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
Medium	Other medium: Base medium: Nutristem medium Main protein source: Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
TRA 1-60	Yes
TRA 1-81	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	No
Other Genotyping (Cell Line)