NCH78 B2-F2
The cell line is not validated yet.
NCHi009-A-1
General
iPSC Line |
|
| hPSCreg name | NCHi009-A-1 |
| Cite as: | NCHi009-A-1 |
| Alternative name(s) |
NCH78 B2-F2
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 10th August 2026 |
| User feedback | |
Provider |
|
| Generator |
Nationwide Children's Hospital (NCH)
Contact:
Nationwide Children's Hospital (NCH) |
| Owner | Nationwide Children's Hospital (NCH) |
| Distributors | |
| Derivation country | United States |
External iPSC Databases |
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| BioSamples | SAMEA122376113 |
General iPSC Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
iPSC Genetic Modification
| Disease/phenotype related modifications |
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Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Family history | Parents were asymptomatic for HLHS |
| Is the medical history available upon request? | Yes |
| Is clinical information available? | Yes |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA112359099 |
Ethics
Also have a look at the ethics information for the parental line
NCHi009-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | ThermoFisher Scientific |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | Must request from owner. |
iPSC Derivation
General |
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The source cell information can be found in the parental cell line
NCHi009-A.
|
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| Passage number reprogrammed | 15 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Selection criteria for clones | Morphology and colony formation |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
Differentiation Potency
In vitro directed differentiation
Morphology
In vitro directed differentiation
Morphology
In vitro directed differentiation
Morphology
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
Yes
46,XX
Passage number: 30
Karyotyping method:
Low-pass whole genome sequencing
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Other Genotyping (iPSC Line) |
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