NERCi001-A
General
Cell Line |
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| hPSCreg name | NERCi001-A |
| Cite as: | NERCi001-A (RRID:CVCL_A0YM) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 2nd July 2021 |
| User feedback | |
Provider |
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| Generator | National Engineering and Research Center of Human Stem Cell (NERC) |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA7851605 |
| Cellosaurus | CVCL_A0YM |
| Wikidata | Q108821026 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Family history | Brother was diagnosed as having intrauterine growth retardation and cerebellar dysplasia. He could not walk until he was 3 years of age. At age 5, he was presented with repeated subcutaneous hemorrhage in the neck and lower extremities and upper respirato |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA7851606 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Has the donor agreed to be re-contacted? | No |
| Has the donor been informed about how her/his data will be protected? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
An undifferentiated stromal cell with the ability to develop into the cells that form distinct mesenchymal tissues; such as bone, muscle, connective tissue, blood vessels, and lymphatic tissue.
Synonyms
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| Source cell origin |
An organ present in some vertebrates during embryonic gestation that surrounds the fetus and provides it with nutrients and oxygen, facilitates gas and waste exchange between the fetus and mother, and provides parasitic cloaking from the mother's immune system by excretion of neurokinin B.
Synonyms
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Passage method | Enzymatically |
| Medium |
Other medium:
Base medium: DMEM-F12
Main protein source: Fetal bovine serum Serum concentration: 10 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| CD90 |
Yes |
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| CD73 |
Yes |
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| CD105 |
Yes |
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| CD45 |
No |
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| CD11b |
No |
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| CD19 |
No |
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| CD34 |
No |
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| HLA-DR |
No |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Self-renewal
Positive
Endoderm
Unknown
Mesoderm
Positive
Ectoderm score
Unknown
In vitro adipogenic induction, a large amount of cytoplasm was stained red with oil red O. In vitro osteogenesis induction, cells were stained dark purple with osteogenic detection staining solution BCIP / NBT. These indicate that DC-PMSCs have adipogenic and osteogenic abilities, the differentiation potential of mesodermal stem cells.
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XY
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Other Genotyping (Cell Line) |
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