General#

Cell Line

hPSCreg Name PCIi002-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd November 2018
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Provider

Generator PHENOCELL (PCI)
Contact:
PHENOCELL (PCI)
Owner Medical University of Vienna (MUW)
Derivation country France

External Databases

BioSamples SAMEA5248238

General Information

Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: R-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5248239

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms
Source cell origin
Blood
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Unkown

Vector free reprogramming

Other

Selection criteria for clones Morphologie, associated to analysis of surface markers (SSEA-3, TRA-1-81), nuclear markers (OCT4, NONOG, SOX2) and spontaneous formation of derivatives of the three germ layers (EB).
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Laminin
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 4 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 - OCT4
Yes
SOX2
Yes
NANOG
Yes
TRA 1-81
Yes
SSEA-4
Yes
Morphology pictures
Spontaneous formation of embryoid bodies and differentiation into derivatives of the three germ layers.
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
GATA6
Yes
EOMES
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
P63
Yes
PAX6
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: R-Banding

Other Genotyping (Cell Line)