General

iPSC Line
hPSCreg name	PCIi033-A-6
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	PCIi033-A-6
Alternative name(s)	IMRB-CFTR-Y122X
iPSC line type	Human induced pluripotent stem cell (hiPSC)
Similar iPSC lines	No similar lines found.
Last update	9th March 2026
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Provider
Generator	Institut Mondor de Recherche Biomédicale (IMRB)
Owner	Institut Mondor de Recherche Biomédicale (IMRB)
Distributors	Institut Mondor de Recherche Biomédicale (IMRB)
Derivation country	France
External iPSC Databases
BioSamples	SAMEA120077266
General iPSC Information
* Is the cell line readily obtainable for third parties?	No
Subclone of	PCIi033-A

iPSC Genetic Modification

Disease/phenotype related modifications

Cystic Fibrosis Synonyms Cystic Fibrosis cystic fibrosis Genetic modifications CFTR (target) Type of Modification Isogenic modification Chromosome location 7q31.2 Nucleotide sequence variant in HGVS format NM_000492.4(CFTR):c.366T>A (p.Tyr122Ter) Protein sequence variant in HGVS format NP_000483.3:p.Tyr122Ter Is the modification homozygous or heterozygous? Homozygous How has the target locus been modified? Mutated

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Is the medical history available upon request?	No
Is clinical information available?	No
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
External Databases (Donor)
BioSamples	SAMEA9699309

Ethics

Also have a look at the ethics information for the parental line PCIi033-A .

Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?	Synthego and IDT
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above?	Yes
Constraints for use or distribution	Phenocell. Distribution of the cell line can be done for academic use only

iPSC Derivation

General
The source cell information can be found in the parental cell line PCIi033-A.
Reprogramming method
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

iPSC Culture Conditions

Surface coating	Vitronectin
Feeder cells	No
Passage method	Enzyme-free cell dissociation Gentle Cell Dissociation Reagent
O2 Concentration	95 %
CO2 Concentration	5 %
Medium	mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line?	Yes

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Oct4	Yes
SOX2	Yes
NANOG	Yes
TRA 1-81	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro directed differentiation

Marker	Expressed
GATA4	Yes
SOX17	Yes

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro directed differentiation

Marker	Expressed
CXCR4	Yes
Brachyury	Yes

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro directed differentiation

Marker	Expressed
Nestin	Yes
Pax6	Yes

Microbiology / Virus Screening
Mycoplasma	Negative

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?	Yes 46,XY Karyotyping method: mFISH
Other Genotyping (iPSC Line)
Is there genome-wide genotyping or functional data available?	Yes SNP typing array