IMRB-CFTR-c.1680-886A>G
PCIi033-A-9
General
iPSC Line |
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| hPSCreg name | PCIi033-A-9 |
| Cite as: | PCIi033-A-9 |
| Alternative name(s) |
IMRB-CFTR-c.1680-886A>G
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 9th March 2026 |
| User feedback | |
Provider |
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| Generator | Institut Mondor de Recherche Biomédicale (IMRB) |
| Owner | Institut Mondor de Recherche Biomédicale (IMRB) |
| Distributors | |
| Derivation country | France |
External iPSC Databases |
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| BioSamples | SAMEA120132842 |
General iPSC Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
iPSC Genetic Modification
| Disease/phenotype related modifications |
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Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
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| BioSamples | SAMEA9699309 |
Ethics
Also have a look at the ethics information for the parental line
PCIi033-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | IDT, Synthego |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | Phenocell. Distribution of the cell line can be done for academic use only |
iPSC Derivation
General |
|
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The source cell information can be found in the parental cell line
PCIi033-A.
|
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Reprogramming method |
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
iPSC Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| O2 Concentration | 95 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| GATA4 |
Yes |
| SOX17 |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| CXCR4 |
Yes |
| Brachyury |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| Pax6 |
Yes |
| Nestin |
Yes |
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
mFISH
|
Other Genotyping (iPSC Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
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