UCB144-CT2-C DOCK2KO clone 20, Cornelius DOCK2KO clone 20
PMUi002-A-2
General
Cell Line |
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| hPSCreg name | PMUi002-A-2 |
| Cite as: | PMUi002-A-2 (RRID:CVCL_C1VR) |
| Alternative name(s) |
UCB144-CT2-C DOCK2KO clone 20, Cornelius DOCK2KO clone 20
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
PMUi002-A-1 (UCB144-CT2-C DOCK2KO clone 19, Cornelius DOCK2KO clone 19) PMUi002-A-3 (Cornelius DOCK2KO clone 22, UCB144-CT2-C DOCK2KO clone 22) PMUi002-A (Cornelius, UCB144-CT2-C) PMUi002-B (Gustav, UCB144-CT1-G) |
| Last update | 3rd January 2022 |
| User feedback | |
Provider |
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| Generator | Paracelsus Medical University (PMU) |
External Databases |
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| BioSamples | SAMEA11980792 |
| Cellosaurus | CVCL_C1VR |
| Wikidata | Q114312670 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | unknown |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA11459147 |
Ethics
Also have a look at the ethics information for the parental line
PMUi002-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
PMUi002-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line PMUi002-A .Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Disease/phenotype related modifications |
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