PMUi002-A-2

UCB144-CT2-C DOCK2KO clone 20, Cornelius DOCK2KO clone 20

General

Cell Line

hPSCreg Name
PMUi002-A-2
Alternative name(s)
UCB144-CT2-C DOCK2KO clone 20, Cornelius DOCK2KO clone 20
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 3rd January 2022
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Provider

Generator Paracelsus Medical University (PMU)

External Databases

BioSamples SAMEA11980792
CLO CLO_0103720

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA11459147

Ethics

Also have a look at the ethics information for the parental line PMUi002-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line PMUi002-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line PMUi002-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Severe Combined Immunodeficiency
Synonyms
  • Severe Combined Immune Deficiency
  • severe combined immunodeficiency disease
  • Severe Combined Immunodeficiency
  • SCID
show more synonyms
Genetic modifications
DOCK2 dedicator of cytokinesis 2 (target)
Gene knock-out
Cytoband location: 5q35.1
CRISPR-associated (CRISPR/Cas) System