General

Cell Line
hPSCreg name	RCPCMi003-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	RCPCMi003-A (RRID:CVCL_UK96)
Alternative name(s)	ATXN1KCl2
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	29th November 2021
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Provider
Generator	Federal State Budgetary Institution Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency (RCPCM)
External Databases
BioSamples	SAMEA5230329
Cellosaurus	CVCL_UK96
Wikidata	Q98128875
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: allowed Commercial use: allowed

Donor Information

General Donor Information
Sex	female
Ethnicity	Caucasian
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Spinocerebellar ataxia type 1 Spinocerebellar ataxia type 1 The donor is a carrier of a disease-associated mutation and affected.
Karyotyping (Donor)
Has the donor karyotype been analysed?	Yes Karyotyping method: G-Banding
External Databases (Donor)
BioSamples	SAMEA5230330

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	No
Please provide contact information of the holder of the original Donor Information Sheet.	Research Center of Neurology, Deputy Head of Institute S.N Illarioshkin +7 (495) 374-77-76
Do you (Depositor/Provider) hold the original Donor Consent Form?	No
If you do not hold the Donor Consent Form, do you know who does?	Yes
Please provide the contact information	Research Center of Neurology, Deputy Head of Institute S.N Illarioshkin +7 (495) 374-77-76
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent expressly prevent the derivation of pluripotent stem cells?	No
Does consent pertain to a specific research project?	No
Does consent permit unforeseen future research, without further consent?	Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications?	No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
Does consent permit research by
an academic institution?	Yes
a public organisation?	Yes
a non-profit company?	Yes
How may genetic information associated with the cell line be accessed?	Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Federal Scientific Center of Neurology
Approval number	na
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Fibroblast of dermis Any skin fibroblast that is part of some dermis.
Reprogramming method
Vector type	Integrating
Vector	Virus (Lentivirus)
Genes	SOX2 KLF4 MYC
Is the used vector excisable?	Yes
Absence of reprogramming vector(s)?	No
Reprogramming vectors silenced?
Methods used	Immunostaining
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
O2 Concentration	5 %
Medium	TeSR™ E8™ IPSC_lines.pdf
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles

Microbiology / Virus Screening
HIV 1	Negative
HIV 2	Negative
Hepatitis B	Negative
Hepatitis C	Negative
Mycoplasma	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46 Passage number: 3 Karyotyping method: G-Banding
Other Genotyping (Cell Line)