RCe019-A-1
General
Cell Line |
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| hPSCreg name | RCe019-A-1 |
| Cite as: | RCe019-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
RCe019-A (RC-15, RC15) |
| Last update | 10th April 2017 |
| User feedback | |
Provider |
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| Generator | Roslin Cells (RC) |
| Owner | Derivation of Clinical Grade hES Cells |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
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General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Ethics
Also have a look at the ethics information for the parental line
RCe019-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
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The source cell information can be found in the parental cell line
RCe019-A.
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Culture Conditions
| Surface coating | Matrigel/Geltrex | |||||||||
| Feeder cells |
dermal fibroblast Cellfinder Ont Id: CL_0002620 |
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| O2 Concentration | 5 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
StemPro® hESC SFM
Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-1 |
No |
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Morphology pictures
Differentiation Potency
Microbiology / Virus Screening |
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 24
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Other Genotyping (Cell Line) |
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