RCe019-A-1
General#
Cell Line |
|
| hPSCreg Name | RCe019-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 10th April 2017 |
| User feedback | |
Provider |
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| Generator | Roslin Cells (RC) |
| Owner | Derivation of Clinical Grade hES Cells |
| Distributors | |
| Derivation country | United Kingdom |
General Information |
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| Is the cell line available in principle? |
Yes |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Ethics#
Also have a look at the ethics information for the parental line
RCe019-A
.
| Alternatives to consent |
| Name of accrediting authority involved? |
| Approval number |
hESC Derivation#
| Date of derivation | 2010-09-30 |
| Embryo stage | Cleavage (Mitosis) |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Expansion status |
5
|
| ICM morphology |
D
|
| Trophectoderm morphology |
g
|
| ZP removal technique | Manual Removal of ZP |
| Cell isolation | Trophoectoderm and ICM isolated |
| Cell seeding | Trophectoderm and ICM seeded onto GMP compataible feeder and xeno-free KOSR media combination |
| Derived under xeno-free conditions? |
Yes |
| Derivation under GMP? |
Yes |
| Available as clinical grade? |
Yes |
Culture Conditions#
| Surface coating | Matrigel/Geltrex | |||||||||
| Feeder cells |
dermal fibroblast Cellfinder Ont Id: CL_0002620 |
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| O2 Concentration | 5 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
StemPro® hESC SFM
Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-1 |
No |
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Morphology pictures
Differentiation Potency
Microbiology / Virus Screening |
|
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
|
Other Genotyping (Cell Line) |
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