General

Cell Line

hPSCreg Name
RCe019-A-1
Cell line type Human embryonic stem cell (hESC)
Last update 10th April 2017
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Provider

Generator Roslin Cells (RC)
Owner Derivation of Clinical Grade hES Cells
Distributors
Derivation country United Kingdom

External Databases

CLO CLO_0102889

General Information

* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics

Also have a look at the ethics information for the parental line RCe019-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line RCe019-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells dermal fibroblast
Cellfinder Ont Id: CL_0002620
O2 Concentration 5 %
CO2 Concentration 5 %
Medium StemPro® hESC SFM
Supplements
BSA 25 %
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 24

Other Genotyping (Cell Line)

Genetic Modification