RCe019-A-1
General#
Cell Line |
|
hPSCreg Name | RCe019-A-1 |
Cell line type | Human embryonic stem cell (hESC) |
Last update | 10th April 2017 |
User feedback | |
Provider |
|
Generator | Roslin Cells (RC) |
Owner | Derivation of Clinical Grade hES Cells |
Distributors | |
Derivation country | United Kingdom |
General Information |
|
Is the cell line available in principle? |
Yes |
Subclone of |
Donor Information#
General Donor Information |
|
Sex | female |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Ethics#
We are currently revising this section.hESC Derivation#
Date of derivation | 2010-09-30 |
Embryo stage | Cleavage (Mitosis) |
Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
PGD Embryo? |
No |
Expansion status |
5![]() |
ICM morphology |
D![]() |
Trophectoderm morphology |
g![]() |
ZP removal technique | Manual Removal of ZP |
Cell isolation | Trophoectoderm and ICM isolated |
Cell seeding | Trophectoderm and ICM seeded onto GMP compataible feeder and xeno-free KOSR media combination |
Derived under xeno-free conditions? |
Yes |
Derivation under GMP? |
Yes |
Available as clinical grade? |
Yes |
Culture Conditions#
Surface coating | Matrigel/Geltrex | |||||||||
Feeder cells |
dermal fibroblast Cellfinder Ont Id: CL_0002620 |
|||||||||
O2 Concentration | 5 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
StemPro® hESC SFM
Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
||||
SSEA-4 |
Yes |
|
||||
TRA 1-60 |
Yes |
|
||||
SSEA-1 |
No |
|
Differentiation Potency
Microbiology / Virus Screening |
|
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46XX
|
Other Genotyping (Cell Line) |
Login to share your feedback, experiences or results with the research community.