SI-211	
    			    			
                RGIe114-A            
            
        General
Cell Line | 
					|
| hPSCreg name | RGIe114-A | 
| Cite as: | RGIe114-A (RRID:CVCL_B632) | 
| Alternative name(s) | 
									 
	SI-211	 
							 | 
						
| Cell line type | Human embryonic stem cell (hESC) | 
| Similar lines | No similar lines found. | 
| Last update | 3rd September 2022 | 
| User feedback | |
Provider | 
					|
| Generator | 
							Reproductive Genetics Institute (RGI)														    								 
    									Contact:
    									 
    																				Stem Cell Research Unit  | 
					
| Derivation country | United States | 
External Databases | 
						|
| Cellosaurus | CVCL_B632 | 
| Wikidata | Q54949987 | 
							    
							General Information | 
					|
| * Is the cell line readily obtainable for third parties? | 
                                     No                                                                      | 
                            
Donor Information
General Donor Information | 
					|
Phenotype and Disease related information (Donor) | 
					|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes | 
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | 
hESC Derivation
| Supernumerary embryos from IVF treatment? | 
								 Yes 
																									
															 | 
						
Culture Conditions
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles | 
| Alkaline Phosphatase | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                ||||
| POU5F1 (OCT-4) | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                
							                 | 
						                |||
| SSEA-3 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                
							                 | 
						                |||
| SSEA-4 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                
							                 | 
						                |||
| TRA 1-60 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                
							                 | 
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| TRA 1-81 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                
							                 | 
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| TRA 2-39 | 
											 Yes 						                 | 
																                
						                
						                	 | 
						                
							                 | 
						                
Genotyping
Karyotyping (Cell Line) | 
					|
| Has the cell line karyotype been analysed? | 
								 Yes 								
																	
											Passage number: 10											 
																													 | 
						
Other Genotyping (Cell Line) | 
					|

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